Immunofluorescence staining of HepG2 cells diluted at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
GCK / Germinal Center Kinase Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C680818-50
Catalog Number: LS-C680818
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Germinal Center Kinase antibody LS-C680818 is an unconjugated rabbit polyclonal antibody to human Germinal Center Kinase (GCK). Validated for ELISA, IF and IHC.
- Application
- ELISA, IF, IHC, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BL44 | GC kinase | Rab8 interacting protein | RAB8IP | MAP4K2 | Rab8-interacting protein | Germinal center kinase | MEK kinase kinase 2 | MEKKK 2
- UniProt Number
- Q12851
- NCBI GenBank
- NM_004579 | NP_004570.2
- SwissProt
- Q12851
Target
- Target
- Human GCK / Germinal Center Kinase
- Antigen Species
- Human
- Gene Name
- MAP4K2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human Mitogen-activated protein kinase kinase kinase kinase 2 protein (386-469AA)
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- 0.01 M PBS