Immunoprecipitation(IP) of GBE1 by using monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-GBE1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

GBE1 Antibody (clone OTI3B4)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$443.00each

Catalog Number: LS-C114876-100

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Overview

Description: GBE1 antibody LS-C114876 is an unconjugated mouse monoclonal antibody to human GBE1. Validated for IHC, IP and WB.
Application: IHC, IHC-P, IP, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: APBD | Brancher enzyme | GBE | Glycogen-branching enzyme | GSD4 | GBE1 | Glycogen branching enzyme
Recommended Dilution: IHC-P: 1:50
Recommended Dilution: WB: 1:2000
UniProt Number: Q04446
NCBI GenBank: NM_000158 | NP_000149.3
SwissProt: Q04446

Target

Target: Human GBE1
Antigen Species: Human
Epitope: Human GBE1
Gene Name: GBE1

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: OTI3B4
Isotype: IgG2a

Immunogen

Immunogen: Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.
Immunogen Type: Full-Length Recombinant Protein