LSBio
GABRA5 Antibody (clone S415-24, APC)

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-A Receptor Alpha5 Antibody. (D) Composite.

GABRA5 Antibody (clone S415-24, APC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$488.00each
Catalog Number: LS-C773943-100

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Overview

Description
GABRA5 antibody LS-C773943 is an APC-conjugated mouse monoclonal antibody to GABRA5 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Application
IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Alpha 5 | GABRA5 | GABA A receptor alpha 5
Recommended Dilution: WB
1:1000
UniProt Number
P31644
SwissProt
P31644

Target

Target
Human GABRA5
Antigen Species
Human
Epitope
Detects ~55 kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
Gene Name
GABRA5

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
S415-24
Isotype
IgG1
Origin Species
Human

Immunogen

Immunogen
Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-Terminus) of human GABA A Receptor Alpha5.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Allophycocyanin
Format
Liquid
Formulation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.