Immunofluorescence staining of FOXA1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse FOXA1 polyclonal antibody (dilution ratio 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

FOXA1 Antibody (Preservative Free)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Mouse
Format:	Liquid

$309.00each

Catalog Number: LS-C820403-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: FOXA1 antibody LS-C820403 is an unconjugated rabbit polyclonal antibody to mouse FOXA1. Validated for ICC, IF, IHC and WB.
Application: ICC, IF, IHC-P, WB
Reactivity: Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Forkhead box A1 | Forkhead box protein A1 | HNF-3-alpha | HNF-3A | TCF-3A | TCF3A | FOXA1 | HNF3A | Transcription factor 3A
Recommended Dilution: IF/ICC: 1:1500 - 1:50000
Recommended Dilution: IHC-P: 1:1000 - 1:5000
Recommended Dilution: WB: 1:500 - 1:1000
UniProt Number: P55317
NCBI GenBank: NM_004496 | NP_004487.2
SwissProt: P55317

Target

Target: Mouse FOXA1
Antigen Species: Mouse
Epitope: Mouse FOXA1
Gene Name: FOXA1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: A synthetic peptide corresponding to the center region of the Mouse FOXA1
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.2 µm filtered solution in PBS
Volume: 100 Microliter
Preservative: No
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS