Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 4A3. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
FOS / c-FOS Antibody (clone 4A3)
| Antibody: | Mouse Monoclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
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- Description
- C-FOS antibody LS-C777324 is an unconjugated mouse monoclonal antibody to human c-FOS (FOS). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Activator protein 1 | AP-1 | Cellular oncogene c-fos | Cellular oncogene fos | FOS | Proto-oncogene c-Fos | v-fos | C-FOS | G0s7 | p55
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P01100
- NCBI GenBank
- NM_005252, NP_005243.1
- SwissProt
- P01100
Target
- Target
- Human FOS / c-FOS
- Antigen Species
- Hu
- Epitope
- Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
- Gene Name
- FOS
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 4A3
- Isotype
- IgG2
- Origin Species
- Hu
Immunogen
- Immunogen
- Synthetic peptide from the N-Terminus of Human c-Fos (aa. 280-380)
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS