Immunohistochemistry with rabbit anti fibronectin biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). Primary antibody: 2 ug/ml for 1 hour @ room T. Secondary antibody: Streptavidin. Conj. HRP 10 ug/ml circa 45 min. @ room T. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FN1 / Fibronectin Antibody (Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Bovine, Human, Mouse, Non-Human Primate, Rat |
| Format: | Lyophilized |
$571.00each
Catalog Number: LS-C60153-100
Catalog Number: LS-C60153
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Fibronectin antibody LS-C60153 is a biotin-conjugated rabbit polyclonal antibody to Fibronectin (FN1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Bov, Hu, Ms, NHP, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CIG | Cold-insoluble globulin | Fibronectin 1 | FINC | FN1 | LETS | GFND | ED-B | Fibronectin | FN | FNZ | GFND2 | Migration-stimulating factor | MSF
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:5000
- UniProt Number
- P02751
- NCBI GenBank
- NM_002026 | NP_002017.1
- SwissProt
- P02751
Target
- Target
- Human FN1 / Fibronectin
- Antigen Species
- Human
- Epitope
- Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
- Gene Name
- FN1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl