Immunofluorescence Microscopy of Mouse Anti-Fluorescein antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: FITC anti-transferrin receptor antibody. Secondary antibody: CF®640R anti-fluorescein secondary antibody at 1ug/mL (left) and 2µg (right) for 45 min at RT. Localization/Staining: plasma membrane and endosome.

Fluorescein Antibody (clone 8B9.C6.D3)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot
Format:	Liquid

$408.00each

Catalog Number: LS-C153772-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Fluorescein antibody LS-C153772 is an unconjugated mouse monoclonal antibody to Fluorescein. Validated for ELISA, Flow, IF, IHC and WB.
Application: ELISA, FC, IF, IHC, WB
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA: 1:5000 - 1:30000
Recommended Dilution: IHC: 1:250 - 1:1500
Recommended Dilution: WB: 1:500 - 1:3000

Target

Target: Fluorescein

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 8B9.C6.D3
Isotype: IgG2a
Light Chain: Kappa Light Chain

Immunogen

Immunogen: Fluorescein conjugated to Mouse IgG.

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Short term: 4°C. Long term: Aliquot and add glycerol (40-50%). Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl