Detection of luciferase expression in CD34+ cells by immunohistochemistry. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with monoclonal anti-luciferase antibody. Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue. Non-transduced, cultured CD34+ cells were used as a negative control. Original magnification, x 40. Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102(10): 3478-3482, 2003.