Flow cytometry of KG1 cells with Mouse anti-Human Fibroblasts/ Epithelial cells. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fibroblasts + Epithelial Cells Antibody (clone D7-FIB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunohistochemistry, Frozen, Immunoprecipitation |
| Reactivity: | Human |
| Format: | Liquid |
$455.00each
Catalog Number: LS-C188124-0.2
Catalog Number: LS-C188124
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- Description
- Fibroblasts + Epithelial Cells antibody LS-C188124 is an unconjugated mouse monoclonal antibody to human Fibroblasts + Epithelial Cells. Validated for Flow, IHC and IP.
- Application
- FC, IHC-F, IP
- Reactivity
- Hu
- Nonreactive Species
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: FC
- 1:50 - 1:200
- Recommended Dilution: IHC-F
- 1:100
Target
- Target
- Human Fibroblasts + Epithelial Cells
- Antigen Species
- Human
- Epitope
- Recognizes a ~112kD molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies, has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. Is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes. Is routinely tested in flow cytometry on the KG1 cell line.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- D7-FIB
- Isotype
- IgG2a
Immunogen
- Immunogen
- Human foreskin fibroblasts.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS