Antibody staining FEV in human small intestine sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
FEV / PET1 Antibody (aa17-49)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C392773-200
Catalog Number: LS-C392773
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- Description
- PET1 antibody LS-C392773 is an unconjugated rabbit polyclonal antibody to human PET1 (FEV) (aa17-49). Validated for IHC, Peptide-ELISA and WB.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- FEV | Fifth Ewing variant protein | HSRNAFEV | PET-1 | PC12 ETS factor 1 | Protein FEV | FEV (ETS oncogene family) | FEV (fifth Ewing variant) | PET1
- Recommended Dilution: IHC-P
- 1:25
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- Q99581
- NCBI GenBank
- NM_017521 | NP_059991.1
- SwissProt
- Q99581
Target
- Target
- Human FEV / PET1
- Antigen Species
- Human
- Epitope
- This FEV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 17-49 amino acids from human FEV.
- Gene Name
- FEV
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 0.5 mg/mL
- Volume
- 200 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS