Strip blots of yeast protein extracts stained with the indicated antibodies. TargetName] antibody is first lane on the left and stains a single band at ~34kDa.
FBL / FIB / Fibrillarin Antibody (clone 38F3)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Rat |
| Format: | Liquid |
$384.00each
Catalog Number: LS-C204517-500
Catalog Number: LS-C204517
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- Description
- Fibrillarin antibody LS-C204517 is an unconjugated mouse monoclonal antibody to Fibrillarin (FBL / FIB) from yeast. It is reactive with human and rat. Validated for ICC, IF and WB.
- Application
- ICC, IF, IHC, WB
- Reactivity
- Hu, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- FBL | FLRN | FBRL | FIB1 | Fibrillarin | RNU3IP1 | FIB
- Recommended Dilution: IF/ICC
- 1:1000 - 1:5000|0.736111111111111 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P22087
- NCBI GenBank
- NM_001436 | NP_001427.2
- SwissProt
- P22087
Target
- Target
- Yeast FBL / FIB / Fibrillarin
- Antigen Species
- Fungus / Yeast / Mold
- Epitope
- This clone stains a single ~34kDa band on western blotting and shows a clear and strong punctate staining of yeast nuclei. Subsequently it was found that this antibody recognizes fibrillarin in a wide variety of species including human, rat, Drosophila, S. pombe, C. elegans, and plants. It can therefore be used to identify nucleoli immunocytochemically.
- Gene Name
- FBL
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 38F3
- Isotype
- IgG1
Immunogen
- Immunogen
- Mice were injected with yeast nuclear preparations and hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tissue Culture Supernatant, 10 mM Sodium Azide
- Volume
- 500 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.