Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min) using Ezrin (Ab-353) antibody (Line 1 and 2) and Ezrin (phospho-Tyr353) antibody (Line 3 and 4).
EZR / Ezrin Antibody (p353)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C826228-100
Catalog Number: LS-C826228
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- Description
- Ezrin antibody LS-C826228 is an unconjugated rabbit polyclonal antibody to Ezrin (EZR) (p353) from human. It is reactive with human and mouse. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CVIL | Cytovillin | CVL | EZR | p81 | VIL2 | Villin 2 (ezrin) | Villin-2 | Cytovillin 2 | Ezrin
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P15311
- NCBI GenBank
- NM_003379 | NP_003370.2
- SwissProt
- P15311
Target
- Target
- Human EZR / Ezrin
- Antigen Species
- Human
- Epitope
- Ezrin (Ab-353) Antibody detects endogenous levels of total Ezrin protein.
- Gene Name
- EZR
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl