Western Blot of rabbit anti-Ezh1 antibody. Marker: Opal Pre-stained ladder Lane 1: NIH/3T3 WCL p/n W10-000-358. Lane 2: NIH/3T3 nuclear extract p/n W10-001-A74. Load: 10 µg per lane. Primary antibody: Ezh1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 60 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 86 kDa for Ezh1 in NIH/3T3 nuclear extract p/n W10-001-A74.
EZH1 / ENX-2 Antibody (Internal)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745408-25
Catalog Number: LS-C745408
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- Description
- ENX-2 antibody LS-C745408 is an unconjugated rabbit polyclonal antibody to ENX-2 (EZH1) (Internal) from human. It is reactive with human and mouse. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Mouse, Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ENX-2 | EZH1 | KIAA0388 | Enhancer of zeste homolog 1 | KMT6B
- Recommended Dilution: ELISA
- 1:50000
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- Q92800
- NCBI GenBank
- NM_001991 | NP_001982.2
- SwissProt
- Q92800
Target
- Target
- Human EZH1 / ENX-2
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest reactivity with this protein in mouse, bovine, and orangutan species based on 100% homology for the immunogen sequence.
- Gene Name
- EZH1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl