Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti-Esrp-1/2 antibody by western blot shows detection in 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
Esrp-1/2 Antibody (clone 23A7.C9)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745257-25
Catalog Number: LS-C745257
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- 2 antibody LS-C745257 is an unconjugated mouse monoclonal antibody to mouse 2 (Esrp-1). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Ms
- Predicted Reactivity
- Bov, Can, Hr, Hu, Mml, NHP, Por, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:750000
- Recommended Dilution: WB
- 1:1000
Target
- Target
- Mouse Esrp-1/2
- Antigen Species
- Ms
- Epitope
- This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 23A7.C9
- Isotype
- IgG2b
- Light Chain
- κ
Immunogen
- Immunogen
- Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
- Immunogen Type
- Fusion-P
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
