Immunofluorescence staining of ER beta/ESR2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ER beta/ESR2 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
ESR2 / ER Beta Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence |
| Reactivity: | Human |
| Format: | Liquid |
$212.00each
Catalog Number: LS-C815589-50
Catalog Number: LS-C815589
For bulk orders or custom formulations:
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- Description
- ER Beta antibody LS-C815589 is an unconjugated rabbit polyclonal antibody to human ER Beta (ESR2). Validated for ICC and IF.
- Application
- ICC, IF
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ESTRB | ER Beta | ERbeta | ESR2 | ER-BETA | ESR-BETA | ESRB | Estrogen receptor 2 (ER beta) | NR3A2 | Estrogen receptor beta | Estrogen receptor beta 4
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- UniProt Number
- Q92731
- NCBI GenBank
- NM_001437 | NP_001428.1
- SwissProt
- Q92731
Target
- Target
- Human ESR2 / ER Beta
- Antigen Species
- Human
- Epitope
- Human ER beta
- Gene Name
- ESR2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the N-Terminus of Human ER beta/ESR2
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 50 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS