LSBio
Escherichia coli O157 Antibody (Preservative Free, Biotin)

The Purity and binding activity of recombinant antibody, anti-O157:H7 by Dot-blotting assay. A. O157 scFv binding activity. Directly spotting O157 antigen into membrane in Row I and II with two dilution (100 and 10 ng of each spot 2-3 ) plus positive system control in spotting blot 1 and 5 as well as BSA control in Spot 4, respectively, then probe the membrane by using O157:H7-specific recombinant antibody after blocking and following anti-Myc-tag and HRP-conjugated detection antibody. B. Checking purity of purified O157 scFv. Directly probed blocking blot with anti-Myctag and following HRP-conjugated detection antibodies.

Escherichia coli O157 Antibody (Preservative Free, Biotin)

Primary AntibodyLSBio Guarantee
Antibody:Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:E. coli
Format:Lyophilized
See all E. coli Escherichia coli O157 antibodies →
$350.00each
Catalog Number: LS-C343560-50

For bulk orders or custom formulations:

Request a Quote

Overview

Description
Escherichia coli O157 antibody LS-C343560 is a biotin-conjugated e. coli polyclonal antibody to escherichia coli Escherichia coli O157. Validated for DB, ELISA and WB.
Application
DB, ELISA, WB
Reactivity
E.col
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:1000 - 1:2000

Target

Target
E. coli Escherichia coli O157
Antigen Species
E. coli
Epitope
It specifically detects protein derived from testing bacterial sample at various concentrations. See image Below.

Antibody

Clonality
Polyclonal

Immunogen

Immunogen
Lyophilized lipopolysaccharide(LPS) isolated from Escherichia Coli O157:H7.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from PBS, No preservatives added
Preservative
No
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: Store at 4 °C for 1 month. Long term: Store at -20°C to -70°C for up to 1 year. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.