Western blot analysis of extracts from SK-OV3 cells using HER2 (Ab-1221/1222) antibody (Line 1 and 2) and HER2 (phospho-Tyr1221/Tyr1222) antibody (Line 3 and 4).
ERBB2 / HER2 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C826262-100
Catalog Number: LS-C826262
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- Description
- HER2 antibody LS-C826262 is an unconjugated rabbit polyclonal antibody to human HER2 (ERBB2). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-erb B2/neu protein | CD340 | HER2 | Herstatin | MLN 19 | HER-2 | Neu | NGL | Proto-oncogene c-ErbB-2 | Proto-oncogene Neu | CD340 antigen | ERBB2 | MLN19 | p185erbB2 | TKR1
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P04626
- NCBI GenBank
- NM_004448 | NP_004439.2
- SwissProt
- P04626
Target
- Target
- Human ERBB2 / HER2
- Antigen Species
- Human
- Epitope
- HER2 (phospho-Tyr1221/Tyr1222) antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine 1221/1222.
- Gene Name
- ERBB2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine1221/1222 (N-L-YP-YP -W).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl