Fluorescent confocal image of SY5Y cells stained with ERAS (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ERAS (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.

ERAS Antibody (aa51-81)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$401.00each

Catalog Number: LS-C98210-400

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: ERAS antibody LS-C98210 is an unconjugated rabbit polyclonal antibody to human ERAS (aa51-81). Validated for IF, IHC and WB.
Application: IF, IHC, IHC-P, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: ES cell expressed Ras | E-Ras | GTPase ERas | HRASP | HRAS2 | ERAS | Small GTPase protein E-Ras
Recommended Dilution: IF/ICC: 1:100
Recommended Dilution: IHC-P: 1:10 - 1:50
Recommended Dilution: WB: 1:1000
UniProt Number: Q7Z444
NCBI GenBank: NP_853510.1
SwissProt: Q7Z444

Target

Target: Human ERAS
Antigen Species: Human
Epitope: This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-81 amino acids from the N-terminal region of human ERAS.
Gene Name: ERAS

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Volume: 400 Microliter
Preservative: Yes
Purification Method: Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer: PBS