LSBio
ER Alpha / Estrogen Receptor Antibody

Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~66.2 kDa ESR1 protein using Rabbit Anti-ESR1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ESR1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~66.2 kDa. Other Band(s): 50 kDa is isoform 2.

ER Alpha / Estrogen Receptor Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human ER Alpha / Estrogen Receptor antibodies →
$451.00each
Catalog Number: LS-C774141-100

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Overview

Description
Estrogen Receptor antibody LS-C774141 is an unconjugated rabbit polyclonal antibody to human Estrogen Receptor (ER Alpha). Validated for IF, IHC and WB.
Application
IF, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Era | ERalpha | Estrogen receptor | ER-alpha | Estrogen receptor 1 | NR3A1 | ER | ESR | ESR1 | ESRA | Estrogen receptor alpha
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: IHC
1:50
Recommended Dilution: WB
1:1000
UniProt Number
P03372
NCBI GenBank
NM_000125 | NP_000116.2
SwissProt
P03372

Target

Target
Human ER Alpha / Estrogen Receptor
Antigen Species
Human
Epitope
Detects ~66 kDa.
Gene Name
ESR1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from the mid-protein of Human ESR1
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.