Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
EP300 / p300 Antibody (aa1000-1050)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287257-10
Catalog Number: LS-C287257
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- Description
- P300 antibody LS-C287257 is an unconjugated rabbit polyclonal antibody to human p300 (EP300) (aa1000-1050). Validated for IHC, IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- E1A binding protein p300 | EP300 | Histone acetyltransferase p300 | KAT3B | p300 HAT | E1A-associated protein p300 | E1A-binding protein, 300kD | p300 | RSTS2
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:1000 - 1:10000
- UniProt Number
- Q09472
- NCBI GenBank
- NM_001429 | NP_001420.2
- SwissProt
- Q09472
Target
- Target
- Human EP300 / p300
- Antigen Species
- Human
- Epitope
- Region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).
- Gene Name
- EP300
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 2033 corresponding to amino acids between 1000 and 1050 of UniprotID: Q09472
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate