Western Blot: The extracts of mouse brain (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ENO2 / NSE Antibody (clone AT17D10)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C200856-50
Catalog Number: LS-C200856
For bulk orders or custom formulations:
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- Description
- NSE antibody LS-C200856 is an unconjugated mouse monoclonal antibody to human NSE (ENO2). Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Enolase 2 | Enolase 2 (gamma, neuronal) | ENO2 | Neurone-specific enolase | Gamma-enolase | Neural enolase | Neuron specific gamma enolase | Neuron-specific enolase | NSE
- Recommended Dilution: WB
- 1:500 - 1:5000
- UniProt Number
- P09104
- NCBI GenBank
- NM_001975 | NP_001966.1
- SwissProt
- P09104
Target
- Target
- Human ENO2 / NSE
- Antigen Species
- Human
- Epitope
- Human ENO2 / NSE
- Gene Name
- ENO2
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AT17D10
- Isotype
- IgG2b
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Recombinant human NSE (1-434aa) purified from E. coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS