LSBio
EN2 Antibody (C-Terminus)

Anti-EN2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

EN2 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$383.00each
Catalog Number: LS-B3477-100

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Overview

Description
EN2 antibody LS-B3477 is an unconjugated rabbit polyclonal antibody to human EN2 (C-Terminus). Validated for IHC and WB. Cited in 1 publication.
Application
IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AUTS10 | Engrailed homolog 2 | Engrailed homeobox 2 | EN2 | Homeobox protein engrailed-2 | Engrailed-2 | Homeobox protein en-2 | Hu-En-2
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
0.05 µg/ml
UniProt Number
P19622
SwissProt
P19622

Target

Target
EN2
Antigen Species
Human
Specificity
Human EN2
Epitope
Human EN2
Gene Name
EN2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from C-Terminus of human EN2 (P19622, NP_001418). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Horse, Pig, Guinea pig (100%); Bat, Opossum, Zebra finch, Chicken, Platypus (92%); Xenopus, Zebrafish (84%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% sodium azide, 2% sucrose
Concentration
0.5 mg/mL
Volume
100 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS
Engrailed-2 (EN2) - a novel biomarker in epithelial ovarian cancer. McGrath SE, Annels N, Madhuri TK, Tailor A, Butler-Manuel SA, Morgan R, Pandha H, Michael A. BMC cancer. 2018 October;18:943. PubMed: 30285763 PMC: PMC6171236

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