LSBio
EN2 Antibody (C-Terminus)

Anti-EN2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

EN2 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$383.00each
Catalog Number: LS-B3477-100

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Overview

Description
EN2 antibody LS-B3477 is an unconjugated rabbit polyclonal antibody to human EN2 (C-Terminus). Validated for IHC and WB. Cited in 1 publication.
Application
IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AUTS10 | Engrailed homolog 2 | Engrailed homeobox 2 | EN2 | Homeobox protein engrailed-2 | Engrailed-2 | Homeobox protein en-2 | Hu-En-2
UniProt Number
P19622
NCBI GenBank
NM_001427 | NP_001418.2
SwissProt
P19622
Recommended Dilution
ApplicationDilution
IHC-P5 µg/ml
WB0.05 µg/ml

Target

Target
EN2
Antigen Species
Human
Specificity
Human EN2
Epitope
Human EN2
Gene Name
EN2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from C-Terminus of human EN2 (P19622, NP_001418). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Horse, Pig, Guinea pig (100%); Bat, Opossum, Zebra finch, Chicken, Platypus (92%); Xenopus, Zebrafish (84%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% sodium azide, 2% sucrose
Concentration
0.5 mg/mL
Volume
100 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS
Engrailed-2 (EN2) - a novel biomarker in epithelial ovarian cancer. McGrath SE, Annels N, Madhuri TK, Tailor A, Butler-Manuel SA, Morgan R, Pandha H, Michael A. BMC cancer. 2018 October;18:943. PubMed: 30285763 PMC: PMC6171236
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