Western blot analysis of extracts of various cell lines, using EMB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

EMB/ Embigin Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human
Format:	Liquid

See all Human EMB/ Embigin antibodies â†’

$281.00each

Catalog Number: LS-C497132-20

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Overview

Description: Embigin antibody LS-C497132 is an unconjugated rabbit polyclonal antibody to human Embigin (EMB). Validated for WB.
Application: WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: EMB | Embigin homolog (mouse) | Embigin | Embigin homolog
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q6PCB8
NCBI GenBank: NM_198449 | NP_940851.1
SwissProt: Q6PCB8

Target

Target: Human EMB/ Embigin
Antigen Species: Human
Gene Name: EMB

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-260 of human EMB (NP_940851.1). DGSAPDSPFTSPPLREEIMANNFSLESHNISLTEHSSMPVEKNITLERPSNVNLTCQFTTSGDLNAVNVTWKKDGEQLENNYLVSATGSTLYTQYRFTIINSKQMGSYSCFFREEKEQRGTFNFKVPELHGKNKPLISYVGDSTVLTCKCQNCFPLNWTWYSSNGSVKVPVGVQMNKYVINGTYANETKLKITQLLEEDGESYWCRALFQLGESEEHIELVVLSYLVP
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)