Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min), using 4E-BP1 (Ab-45) antibody (Line1 and 2) and 4E-BP1 (phospho-Thr45) antibody (Line 3 and 4).
EIF4EBP1 / 4EBP1 Antibody (p45)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C826108-100
Catalog Number: LS-C826108
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- Description
- 4EBP1 antibody LS-C826108 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1) (p45) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 4E-BP1 | 4EBP1 | BP-1 | EIF4EBP1 | PHAS-I | EIF4E-binding protein 1
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q13541
- NCBI GenBank
- NM_004095 | NP_004086.1
- SwissProt
- Q13541
Target
- Target
- Human EIF4EBP1 / 4EBP1
- Antigen Species
- Human
- Epitope
- 4E-BP1 (Ab-45) antibody detects endogenous levels of total 4E-BP1 protein. This antibody may cross-react with 4E-BP2 and 4E-BP3.
- Gene Name
- EIF4EBP1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl