
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~12.6 kDa 4EBP1 protein using Rabbit Anti-4EBP1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-4EBP1 Polyclonal Antibody at 1:500 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~12.6 kDa.
EIF4EBP1 / 4EBP1 Antibody (Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$485.00each
Catalog Number: LS-C774408-100
Catalog Number: LS-C774408
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- Description
- 4EBP1 antibody LS-C774408 is a biotin-conjugated rabbit polyclonal antibody to human 4EBP1 (EIF4EBP1). Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 4E-BP1 | 4EBP1 | BP-1 | EIF4EBP1 | PHAS-I | EIF4E-binding protein 1
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q13541
- NCBI GenBank
- NM_004095 | NP_004086.1
- SwissProt
- Q13541
Target
- Target
- Human EIF4EBP1 / 4EBP1
- Antigen Species
- Human
- Epitope
- Detects 12.6 kDa.
- Gene Name
- EIF4EBP1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the mid-protein of Human 4EBP1 (aa. 50-80)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS
