LSBio
DYKDDDDK Tag Antibody (C-Terminus, HRP)

DYKDDDDK Tag Antibody (C-Terminus, HRP)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Other
Format:Lyophilized
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$608.00each
Catalog Number: LS-C187731-0.1

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Overview

Description
DYKDDDDK Tag antibody LS-C187731 is an HRP-conjugated rabbit polyclonal antibody to synthetic DYKDDDDK Tag (C-Terminus). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Other
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Synthetic DYKDDDDK Tag
Antigen Species
Other
Epitope
Recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. Has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays. In Western blotting of bacterial extractshas been shown not to cross-react with any endogenous proteins.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Keyhole limpet hemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-Terminus end.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
PBS, 0.01% Gentamicin, 1% BSA
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Before reconstitution store at 4°C. After reconstitution store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.