LSBio
Donkey Anti-Sheep IgG Secondary Antibody

ELISA results of purified ELISA of Donkey anti-Sheep IgG Antibody tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Gt-a-Donkey IgG HRP conjugate 1:10,000, and TMB-8000 substrate.

Donkey Anti-Sheep IgG Secondary Antibody

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Other, Western Blot
Reactivity:Sheep
Format:Liquid
See all Sheep IgG antibodies →
$303.00each
Catalog Number: LS-C61145-2

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Overview

Description
Sheep IgG antibody LS-C61145 is an unconjugated donkey polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, IP, Other, WB
Reactivity
Sh
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Sheep IgG
Antigen Species
Sheep
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Sheep IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.