Anti-Sheep IgG Secondary Antibody
Catalog Number: LS-C758137-10
10 mg / $286.00
Antibody: Sheep Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Sheep
Format: Liquid
ELISA results of purified Anti-SHEEP IgG (H&L) (DONKEY) Alkaline Phosphatase Conjugated tested against Sheep IgG. Each well was coated in 1.0 µg of antigen. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer and Substrate
Donkey Anti-Sheep IgG Secondary Antibody (AP)
Secondary AntibodyLSBio Guarantee
| Antibody: | Donkey Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Sheep |
| Format: | Liquid |
$360.00each
Catalog Number: LS-C61149-1
Catalog Number: LS-C61149
For bulk orders or custom formulations:
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- Description
- Sheep IgG antibody LS-C61149 is an AP-conjugated donkey polyclonal antibody. Validated for ELISA, IHC and WB.
- Application
- DB, ELISA, IHC, WB
- Reactivity
- Sh
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: ELISA
- 1:2000 - 1:8000
- Recommended Dilution: IHC
- 1:200 - 1:1000
- Recommended Dilution: WB
- 1:1000 - 1:4000
Target
- Target
- Sheep IgG
- Antigen Species
- Sheep
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum.
Antibody
- Host Species
- Donkey
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Alkaline Phosphatase
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.1% Sodium Azide, 50% (v/v) Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C. Do not freeze.
- Recommended Storage Buffer
- 0.05 M Tris-Chloride|0.15 M NaCl