LSBio
Donkey Anti-Sheep IgG Secondary Antibody (AP)

ELISA results of purified Polyclonal Donkey Anti-Sheep IgG Antibody Alkaline Phosphatase Conjugated tested against Sheep IgG. Each well was coated in duplicate with 1.0µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer and Substrate

Donkey Anti-Sheep IgG Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Sheep
Format:Liquid
See all Sheep IgG antibodies →
$439.00each
Catalog Number: LS-C61142-1

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Overview

Description
Sheep IgG antibody LS-C61142 is an AP-conjugated donkey polyclonal antibody. Validated for ELISA.
Application
DB, ELISA, WB
Reactivity
Sh
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:2000 - 1:10000

Target

Target
Sheep IgG
Antigen Species
Sheep
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, guinea pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Sheep IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Alkaline Phosphatase
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.