LSBio
Donkey Anti-Rabbit IgG Secondary Antibody (TR)

Dot Blot showing the detection of Rabbit IgG. A three-fold serial dilution of Rabbit IgG starting at 200ng was spotted onto 0.45 um nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20C, Anti-Rabbit IgG (H&L) (DONKEY) Antibody Texas Red Conjugated (p/n Anti-RABBIT IgG (H&L) (DONKEY) Antibody Texas Red® Conjugated) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Donkey Anti-Rabbit IgG Secondary Antibody (TR)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Flow Cytometry, Immunofluorescence, Other, Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$324.00each
Catalog Number: LS-C60953-2

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Overview

Description
Rabbit IgG antibody LS-C60953 is a TR-conjugated donkey polyclonal antibody. Validated for FLISA, Flow, IF and WB.
Application
FC, IF, Other, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: FC
1:500 - 1:2500
Recommended Dilution: IF/ICC
1:1000 - 1:5000
Recommended Dilution: Other
FLISA: 1:10000 - 1:50000

Target

Target
Rabbit IgG
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG, and Rabbit Serum.

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Texas Red
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.