LSBio
Donkey Anti-Rabbit IgG Secondary Antibody (RPE)

Western blot of Phycoerythrin conjugated Donkey F(ab')2 Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin donkey secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

Donkey Anti-Rabbit IgG Secondary Antibody (RPE)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$384.00each
Catalog Number: LS-C61493-500

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Overview

Description
Rabbit IgG antibody LS-C61493 is an RPE-conjugated donkey polyclonal antibody. Validated for Flow, IF, IHC and WB.
Application
FC, IF, IHC, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: FC
1:100 - 1:250
Recommended Dilution: IF/ICC
1:100 - 1:200
Recommended Dilution: WB
1:1000

Target

Target
Rabbit IgG
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Fab2 Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole molecule in donkey.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Phycoerythrin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.5 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.