LSBio
Donkey Anti-Rabbit IgG Secondary Antibody (HRP)

Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase donkey secondary antibody at 1:1000 for 60 min at RT Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Donkey Anti-Rabbit IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$340.00each
Catalog Number: LS-C60950-2

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Overview

Description
Rabbit IgG antibody LS-C60950 is an HRP-conjugated donkey polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB. Cited in 1 publication.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:40000
Recommended Dilution: IHC
1:500 - 1:2000
Recommended Dilution: WB
1:1000 - 1:3000

Target

Target
Rabbit IgG
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice. Qianyu Zhang, Zhifu Wei, Huinan Weng, Ye Chen, Jie Zhang, Shiwei Mei, Jiahui Wei, Xiulan Zhu, Yingqi Nong, Jianxing Ruan, Wenjuan Liu, Ruiqiong Zhou, Fang Wang, Yanni Xie, Junjiu Huang, Xiqian Zhang, Fenghua Liu. Frontiers in nutrition. 2022 July;9:854655. PubMed: 35836584 PMC: PMC9274203

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