LSBio
Donkey Anti-Rabbit IgG Secondary Antibody (AP)

Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule Lane 2: Rabbit IgG F(ab) Fragment Lane 3: Rabbit IgG F(c) Fragment Lane 4: Rabbit IgM Whole Molecule Lane 5: Normal Rabbit Serum All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM.

Donkey Anti-Rabbit IgG Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Liquid
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$439.00each
Catalog Number: LS-C60944-1

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Overview

Description
Rabbit IgG antibody LS-C60944 is an AP-conjugated donkey polyclonal antibody. Validated for ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:10000 - 1:20000
Recommended Dilution: IHC
1:400 - 1:2000
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Rabbit IgG
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with Rabbit IgG whole molecule in donkey
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Alkaline Phosphatase
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

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