LSBio
Donkey Anti-Rabbit IgG Fc Secondary Antibody (Biotin)

Western Blot of Unconjugated Anti-Rabbit IgG F(c) (DONKEY) Antibody Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule Lane 2: Rabbit IgG F(ab) Fragment Lane 3: Rabbit IgG F(c) Fragment Lane 4: Rabbit IgM Whole Molecule Lane 5: Normal Rabbit Serum All samples were reduced. Load: 50 ng per lane.

Donkey Anti-Rabbit IgG Fc Secondary Antibody (Biotin)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$360.00each
Catalog Number: LS-C153815-2

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Overview

Description
Rabbit IgG Fc antibody LS-C153815 is a biotin-conjugated donkey polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:300000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:20000

Target

Target
Rabbit IgG Fc
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG F(c) fragment

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.