LSBio
Donkey Anti-Mouse IgG Secondary Antibody (HRP)

Western Blot of Peroxidase Donkey Anti-Mouse IgG Pre-Adsorbed secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.

Donkey Anti-Mouse IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Mouse
Format:Lyophilized
See all Mouse IgG antibodies →
$421.00each
Catalog Number: LS-C60842-1

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Overview

Description
Mouse IgG antibody LS-C60842 is an HRP-conjugated donkey polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:80000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:20000

Target

Target
Mouse IgG
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C while lyophilized, once reconstituted aliquot and freeze at -20°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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