Anti-Goat IgG Secondary Antibody (HRP)
Catalog Number: LS-C170893-1
1 unit / $335.00
Antibody: Goat Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human
Format: Liquid
ELISA results of purified Donkey anti-Goat IgG antibody Peroxidase conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, and TMB substrate
Donkey Anti-Goat IgG Secondary Antibody (HRP)
Secondary AntibodyLSBio Guarantee
| Antibody: | Donkey Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot |
| Reactivity: | Goat |
| Format: | Lyophilized |
$349.00each
Catalog Number: LS-C60366-2
Catalog Number: LS-C60366
For bulk orders or custom formulations:
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- Description
- Goat IgG antibody LS-C60366 is an HRP-conjugated donkey polyclonal antibody. Validated for ELISA, IE, IHC and WB.
- Application
- DB, ELISA, IHC, Other, WB
- Reactivity
- Gt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: ELISA
- 1:10000 - 1:150000
- Recommended Dilution: IHC
- 1:500 - 1:2500
- Recommended Dilution: WB
- 1:1000 - 1:5000
Target
- Target
- Goat IgG
- Antigen Species
- Goat
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum.
Antibody
- Host Species
- Donkey
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-Goat IgG (H&L) was produced by repeated immunization with Goat IgG whole molecule in donkey.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 2 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store lyophilized at 4°C. Following resuspension aliquot and store at -20°C. Avoid freeze-thaw cycles and dilute immediately before use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
