Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 11H8. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) DNMT1 Antibody. (D) Composite.
DNMT / DNMT1 Antibody (clone 11H8)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$444.00each
Catalog Number: LS-C773368-100
Catalog Number: LS-C773368
For bulk orders or custom formulations:
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- Description
- DNMT1 antibody LS-C773368 is an unconjugated mouse monoclonal antibody to DNMT1 (DNMT) from human. It is reactive with human and mouse. Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu, Ms
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AIM | CXXC finger protein 9 | CXXC9 | DNA methyltransferase 1 | DNMT | DNA methyltransferase HsaI | DNA MTase HsaI | HSN1E | M.HsaI | MCMT | DNMT1
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P26358
- NCBI GenBank
- NM_001379, NP_001370.1
- SwissProt
- P26358
Target
- Target
- Human DNMT / DNMT1
- Antigen Species
- Hu
- Epitope
- Detects ~180 kDa. It will cross-react with mouse DNMT1.
- Gene Name
- DNMT1
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 11H8
- Isotype
- IgG1
- Origin Species
- Hu
Immunogen
- Immunogen
- Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1 (Gene accession number NP_001370).
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, 0.05% BSA, 0.05% sodium azide, 50% glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS