Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in ll-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with l-Ptase.
DNM1 / Dynamin Antibody (phospho-Ser774)
Primary AntibodyLSBio Guarantee
| Antibody: | Sheep Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Chicken, Dog, Human, Mouse, Rat |
| Format: | Liquid |
$479.00each
Catalog Number: LS-C241-100
Catalog Number: LS-C241
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- Description
- Dynamin antibody LS-C241 is an unconjugated sheep polyclonal antibody to Dynamin (DNM1) (pSer774) from rat. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Bov, Can, Ck, Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Dynamin | DNM1 | Dynamin-1 | DNM | Dynamin 1 | p-Ser774 Dynamin
- Recommended Dilution: IHC
- 1:1000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q05193
- NCBI GenBank
- NM_004408 | NP_004399.2
- SwissProt
- Q05193
Target
- Target
- Rat DNM1 / Dynamin
- Antigen Species
- Rat
- Epitope
- Specific for the ~95k dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e. g. amphiphysin and synapsin). Immunolabeling is blocked by X-phosphatase treatment.
- Gene Name
- DNM1
Antibody
- Host Species
- Sheep
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A phosphopeptide corresponding to amino acid residues surrounding the phospho Ser774 of rat dynamin.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 10 mM HEPES|150 mM NaCl