Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 µM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Dityrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Dityrosine Antibody (clone 7D4, Atto 488)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:	Pan-species / Cross-reactive
Format:	Liquid

See all All species Dityrosine antibodies â†’

$545.00each

Catalog Number: LS-C821276-100

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Overview

Description: Dityrosine antibody LS-C821276 is an Atto 488-conjugated mouse monoclonal antibody to all species Dityrosine. Validated for ELISA, IF and WB.
Application: ELISA, IF, WB
Reactivity: Pan
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA: 1:1000
Recommended Dilution: IF/ICC: 1:50
Recommended Dilution: WB: 1:1000

Target

Target: All species Dityrosine
Antigen Species: Pan-species / Cross-reactive
Epitope: Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.