Diablo was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg MCF-7 Whole Cell Lysate. Lane C:0.5 mg HepG2 Whole Cell Lysate. 2 uL anti-Diablo rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-Diablo rabbit monoclonal antibody, at 1:330 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa.
DIABLO / SMAC Antibody (clone 001, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Monoclonal |
| Applications: | Immunoprecipitation |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C820670-100
Catalog Number: LS-C820670
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- Description
- SMAC antibody LS-C820670 is an unconjugated rabbit monoclonal antibody to human SMAC (DIABLO). Validated for IP.
- Application
- IP
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- DFNA64 | DIABLO-S | DIABLO | Diablo homolog, mitochondrial | SMAC3 | Mitochondrial Smac protein | SMAC
- UniProt Number
- Q9NR28
- NCBI GenBank
- NM_019887 | NP_063940.1
- SwissProt
- Q9NR28
Target
- Target
- Human DIABLO / SMAC
- Antigen Species
- Human
- Epitope
- Human Smac.
- Gene Name
- DIABLO
Antibody
- Host Species
- Rabbit
- Clonality
- Monoclonal
- Clone
- 001
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human SMAC / Diablo protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS