Western blot of HeLa cell lysate showing specific immunolabeling of the ~142 kDa DENND3 protein phosphorylated at Thr450 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (?-Ptase, 800 units/1mg protein for 30 min).

DENND3 Antibody (phospho-Thr450)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$479.00each

Catalog Number: LS-C772963-100

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Quantity

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Overview

Description: DENND3 antibody LS-C772963 is an unconjugated rabbit polyclonal antibody to human DENND3 (pThr450). Validated for IHC and WB.
Application: IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: DENND3 | KIAA0870 | DENN/MADD domain containing 3
Recommended Dilution: IHC: 1:400
Recommended Dilution: WB: 1:1000
UniProt Number: A2RUS2
SwissProt: A2RUS2

Target

Target: Human DENND3
Antigen Species: Human
Gene Name: DENND3

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr450 conjugated to KLH
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 10 mM HEPES pH7.5, 150 mM NaCl, 50% Glycerol
Volume: 100 Microliter
Preservative: No
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year.
Recommended Storage Buffer: 10 mM HEPES|150 mM NaCl