Blots of crude rat brain extract from a postnatal 3 day animal stained with DCX / Doublecortin antibody. Two bands at ~45 kDa and ~35 kDa show that DCX / Doublecortin antibody binds to an epitope in the region of DCX shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A.
DCX / Doublecortin Antibody (clone 3E1)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Human, Mouse, Pig, Rat |
| Format: | Liquid |
$384.00each
Catalog Number: LS-C204512-100
Catalog Number: LS-C204512
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- Description
- Doublecortin antibody LS-C204512 is an unconjugated mouse monoclonal antibody to Doublecortin (DCX) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
- Application
- ICC, IF, IHC, WB
- Reactivity
- Bov, Hu, Ms, Por, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- DBCN | Doublecortin | Doublin | Doublecortex | Lis-X | SCLH | LISX | XLIS | DCX | Lissencephalin-X
- Recommended Dilution: IF/ICC
- 1:1000|0.736111111111111
- Recommended Dilution: WB
- 1:10000
- UniProt Number
- O43602
- NCBI GenBank
- NM_000555 | NP_000546.2
- SwissProt
- O43602
Target
- Target
- Human DCX / Doublecortin
- Antigen Species
- Human
- Epitope
- Clone 3E1 is known to react with Fox3/NeuN from human, cow, pig, mouse, rat and other mammals. Since Fox3/NeuN is highly conserved, it is likely that the antibody is effective on other species also.
- Gene Name
- DCX
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 3E1
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Recombinant full length human Lis-A isoform of DCX purified from E. coli.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 10 mM Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS