LSBio
CYP27B1 Antibody (aa475-508)

CYP27B1 antibody Western blot. All lanes: Anti CYP27B1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Mouse Kidney Tissue Lysate at 50 ug. Lane 3: 293T Whole Cell Lysate at 40 ug. Predicted band size: 57 kD. Observed band size: 57 kD.

CYP27B1 Antibody (aa475-508)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Lyophilized
See all Human CYP27B1 antibodies →
$480.00each
Catalog Number: LS-C407761-100

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Overview

Description
CYP27B1 antibody LS-C407761 is an unconjugated rabbit polyclonal antibody to CYP27B1 (aa475-508) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Cited in 1 publication.
Application
IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
1alpha(OH)ase | 25-OHD-1 alpha-hydroxylase | Calcidiol 1-monooxygenase | CYP1alpha | CYP27B | Cytochrome P450C1 alpha | Cytochrome p450 27B1 | Cytochrome P450VD1-alpha | p450c1 | VD3 1A hydroxylase | PDDR | VDD1 | CP2B | CYP1 | CYP27B1 | VDDR | VDDRI
UniProt Number
O15528
SwissProt
O15528

Target

Target
Human CYP27B1
Antigen Species
Human
Epitope
Kidney.
Gene Name
CYP27B1

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human CYP27B1 (475-508 aa HFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR), different from the related mouse and rat sequences by six amino acids.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Concentration
0.5 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Recommended Storage Buffer
NaCl
Hemoglobin alters vitamin carrier uptake and vitamin D metabolism in proximal tubule cells: implications for sickle cell disease. Megan L Gliozzi, Youssef Rbaibi, Kimberly R Long, Dario A Vitturi, Ora A Weisz. American journal of physiology. Cell physiology. 2019 November;317:C993-C1000. PubMed: 31509446 PMC: PMC6879883

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