Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2. B4 antibody/1 x107 cell equivalents. Immunoprecipitates were resolved by electrophoresis, transferred to nitrocellulose, and probed with the 7H8.2C12 anti-cytochrome c antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. In addition to the specific 15 kD cytochrome c band immunoprecipitated by 6H2. B4, heavy and light immunoglobulin chains are recognized by the goat anti-mouse secondary antibody.
CYCS / Cytochrome c Antibody (clone 6H2.B4)
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
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- Description
- Cytochrome c antibody LS-C41042 is an unconjugated mouse monoclonal antibody to Cytochrome c (CYCS) from mouse. It is reactive with human, mouse and rat. Validated for Flow, IF, IP and WB. Cited in 2 publications.
- Application
- FC, IF, IHC, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CYC | HCS | THC4 | CYCS | Cytochrome c | Cytochrome c, somatic
- UniProt Number
- P99999
- NCBI GenBank
- NM_018947 | NP_061820.1
- SwissProt
- P99999
Target
- Target
- Mouse CYCS / Cytochrome c
- Antigen Species
- Mouse
- Epitope
- Rat cyt c-OVA
- Gene Name
- CYCS
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 6H2.B4
- Isotype
- IgG1
Immunogen
- Immunogen
- Purified mouse CYCS / Cytochrome c.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
- Concentration
- 0.5 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C.
- Recommended Storage Buffer
- Phosphate-buffered
Molecular characterization of skeletal muscle atrophy in the R6/2 mouse model of Huntington's disease. She P, Zhang Z, Marchionini D, Diaz WC, Jetton TJ, Kimball SR, Vary TC, Lang CH, Lynch CJ. American journal of physiology. Endocrinology and metabolism. 2011;301:E49-61.
PubMed: 21505144 · PMC: PMC3129844
Adenovirus type 5 rupture of lysosomes leads to cathepsin B-dependent mitochondrial stress and production of reactive oxygen species. McGuire KA, Barlan AU, Griffin TM, Wiethoff CM. Journal of virology. 2011;85:10806-13.
PubMed: 21835790 · PMC: PMC3187480