Dot Blot of Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated. Antigen: Row 1 - Cy3 Streptavidin Conjugated Row 2 - Cy5 Streptavidin Conjugated. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.

Cyanine Antibody (AP)

Primary AntibodyLSBio Guarantee

Antibody:	Sheep Polyclonal
Applications:	Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Format:	Liquid

$737.00each

Catalog Number: LS-C154309-1

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Cyanine antibody LS-C154309 is an AP-conjugated sheep polyclonal antibody to Cyanine. Validated for DB, ELISA, IHC and WB.
Application: DB, ELISA, IHC, WB
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:2000 - 1:10000
Recommended Dilution: IHC: 1:200 - 1:1000
Recommended Dilution: WB: 1:500 - 1:2500

Target

Target: Cyanine
Epitope: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.

Antibody

Host Species: Sheep
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: A mixture of Cy3 and Cy5 conjugated KLH.

Format

Conjugate/Label: Alkaline Phosphatase
Format: Liquid
Formulation: 10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified