Immunohistochemistry of rabbit anti-CTCF antibody. Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-CTCF at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: CTCF as precipitated red signal with hematoxylin purple nuclear counterstain.

CTCF Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$311.00each

Catalog Number: LS-C745341-25

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Quantity

For bulk orders or custom formulations:

Overview

Description: CTCF antibody LS-C745341 is an unconjugated rabbit polyclonal antibody to CTCF (C-Terminus) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Hu
Predicted Reactivity: Bovine, Chicken, Horse, Mammal (Other), Mouse
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: 11-zinc finger protein | CCCTC-binding factor | CTCF | Transcriptional repressor CTCF | CTCFL paralog
Recommended Dilution: ELISA: 1:10000 - 1:50000
Recommended Dilution: IHC: 5 µg/ml
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P49711
NCBI GenBank: NM_006565 | NP_006556.1
SwissProt: P49711

Target

Target: Human CTCF
Antigen Species: Human
Epitope: This antibody reacts with endogenous CTCF protein. A BLAST analysis was used to suggest reactivity with CTCF from human, mouse, horse, bovine, panda, rabbit, Danio, and chicken based on a 100% homology with the immunizing sequence. Cross-reactivity with CTCF from other sources has not been determined.
Gene Name: CTCF

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: CTCF affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl