Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
CRYAB / Alpha B Crystallin Antibody (clone OTI5C9)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C114681-100
Catalog Number: LS-C114681
For bulk orders or custom formulations:
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- Description
- Alpha B Crystallin antibody LS-C114681 is an unconjugated mouse monoclonal antibody to human Alpha B Crystallin (CRYAB). Validated for IF, IHC, IP and WB.
- Application
- IF, IHC, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Alpha-crystallin B chain | CRYA2 | Crystallin, alpha B | CTPP2 | Heat shock protein beta-5 | HSPB5 | Alpha B Crystallin | Alpha(B)-crystallin | Rosenthal fiber component | CRYAB | Heat-shock 20 kD like-protein
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:50
- Recommended Dilution: WB
- 1:500
- UniProt Number
- P02511
- NCBI GenBank
- NM_001885 | NP_001876.1
- SwissProt
- P02511
Target
- Target
- Human CRYAB / Alpha B Crystallin
- Antigen Species
- Human
- Epitope
- Human CRYAB / Alpha B Crystallin
- Gene Name
- CRYAB
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI5C9
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 0.81 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS