Western blot analysis of extracts of K562 cells, using Phospho-CRK-Y221 antibody at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.

CRK Antibody (phospho-Tyr221)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$293.00each

Catalog Number: LS-C750628-20

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Overview

Description: CRK antibody LS-C750628 is an unconjugated rabbit polyclonal antibody to CRK (pTyr221) from human. It is reactive with human, mouse and rat. Validated for WB.
Application: WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Adapter molecule crk | CRK | CRKII | Proto-oncogene c-Crk | p38
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P46108
NCBI GenBank: NM_016823 | NP_058431.2
SwissProt: P46108