Fluorescent image of A549 cell stained with CREB1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CREB1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CREB1 immunoreactivity is localized to Nucleus significantly.
CREB1 / CREB Antibody (aa105-132)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C167024-400
Catalog Number: LS-C167024
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- Description
- CREB antibody LS-C167024 is an unconjugated rabbit polyclonal antibody to CREB (CREB1) (aa105-132) from human. It is reactive with human and mouse. Validated for Flow, IF and WB.
- Application
- FC, IF, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CREB-1 | Transactivator protein | CREB | CREB1
- Recommended Dilution: FC
- 1:10 - 1:50
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P16220
- NCBI GenBank
- NM_134442 | NP_004370.1
- SwissProt
- P16220
Target
- Target
- Human CREB1 / CREB
- Antigen Species
- Human
- Epitope
- This CREB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-132 amino acids from the Central region of human CREB1.
- Gene Name
- CREB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS