Western blot analysis of extracts of U-937 cells, using CR1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
CR1 / CD35 Antibody (aa420-550)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C496769-20
Catalog Number: LS-C496769
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- Description
- CD35 antibody LS-C496769 is an unconjugated rabbit polyclonal antibody to human CD35 (CR1) (aa420-550). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C3b/C4b receptor | C3-binding protein | CD35 | C3BR | CR1 | Knops blood group antigen | C4BR | CD35 antigen | Complement receptor type 1 | KN
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P17927
- NCBI GenBank
- NM_000651 | NP_000642.3
- SwissProt
- P17927
Target
- Target
- Human CR1 / CD35
- Antigen Species
- Human
- Gene Name
- CR1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 420-550 of human CR1 (NP_000642.3). FCPSPPVIPNGRHTGKPLEVFPFGKTVNYTCDPHPDRGTSFDLIGESTIRCTSDPQGNGVWSSPAPRCGILGHCQAPDHFLFAKLKTQTNASDFPIGTSLKYECRPEYYGRPFSITCLDNLVWSSPKDVCK
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS